Clinical Investigation

Effects of Vitamin K-3 on Growth Inhibition of Rat glioma Cell (C6) and Human Glioblastoma Multiforme in Vitro

  • Pinar ÖZTOPÇU
  • Selda KABADERE
  • Ruhi UYAR

Received Date: 03.11.2003 Accepted Date: 14.02.2005 Meandros Med Dent J 2005;6(1):21-25

Objectives: Glioblastoma multiforme is characterized as a highly invasive and rapidly growing astrocytoma. Previous studies have reported that vitamin K-3 inhibits cell growth of many types of malignant tumor cell lines. In this study we aimed to investigate the possible inhibitory effect of vitamin K-3 on rat glioma (C6) and human glioblastoma multiforme cells. Method:The cells were exposed to 1, 10, 25, 50, 75 and 100 M of vitamin K-3 for 24 hours. Cell viability was estimated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. For statistical analysis one-way ANOVA test and then Tukey's multiple comparison test were used. Results: The IC50 values of vitamin K-3 were calculated as 41 M for C6, and 24 M and 23 M for human glioblastoma multiforme cells. Conclusion: We have found that at low concentrations vitamin K-3 has an antiproliferative effect on both C6 and human glioma cells. We believe that all these findings should contribute to other in vitro or in vivo studies on vitamin K-3 and glioblastoma multiforme cells.

Keywords: Glioma, vitamin K-3, cell proliferation, in vitro